توضیحات
ائوزین Y کمپانی Bio Optica ایتالیا
محلول ائوزین به عنوان یک رنگ متقابل به همراه هماتوکسیلین در روش رنگ آمیزی هماتوکسیلین – ائوزین H&E مورد استفاده قرار میگیرد. این نوع رنگ آمیزی با استفاده از ظروف شیشه ای یکی از رایج ترین روش های رنگ آمیزی مورد استفاده در بافت شناسی است. در بافتی که با هماتوکسیلین و ائوزین رنگ آمیزی شده باشد، سیتوپلاسم سلول به رنگ صورتی نارنجی و هسته نیز به صورت تیره رنگ، یا به رنگ آبی یا بنفش نشان داده میشود.
• محلول آماده به مصرف
• در بطری پلاستیکی با حجم 500 میلی لیتری
• برای انجام رنگ آمیزی هماتوکسیلین ائوزین H&E
Eosin Y A
Eosin Y is a dry dye for microscopy that is often used as a counterstain, vizualising proteins, connective tissues, fibers and keratin in sample material of human origin. Depending on user requirements and personal preferences, it can be used for the preparation of the commonly used aqueous or alcoholic Eosin Y staining solutions. This staining solution will mostly be used for routine staining in the hematoxylin-eosin staining process (H&E). The H&E staining process is the most frequently used staining method for histological material. Due to the hematoxylin solution, the nuclei will appear blue to dark violet in color and the Eosin Y solution will stain the cytoplasma and intercellular substances red-orange.
Application
Reagent for cytoplasm staining, excellent for extemporary staining.
Eosin Y has been used as a stain:
• to determine the location of the brain sections
• for staining of fixed liver tissue
• for histological examination of brain sections from the dorsal hippocampus
Biochem/physiol Actions
Eosin Y is a xanthene dye and is used for the differential staining of connective tissue and cytoplasm. In histopathology, it is applied as a counterstain after hematoxylin and before methylene blue. It is also used as a background stain, thereby giving contrast to the nuclear stains
Principle:
The Eosin is an acidic dye that interacts with the cytoplasmic proteins rich in basic amino acids to form a complex red-pink brilliant.
The Eosin staining (cytoplasmic staining) in combination with the Hematoxylin (nuclear staining ) is the most used method in the histopathological routine.
Method:
1) Hematoxylin-eosin (for routine staining)
Sections to water
Mayer’s hemalum 5 minutes
Tap water (or Scott’s water for 1 minutes) 5 minutes
Eosin y alcoholic solution 1 minutes
Absolute ethanol 1° 30 seconds
Absolute ethanol 2° 30 seconds
Clearing agent and mount
2) Hematoxylin-eosin (for extemporary staining)
Harris’ hematoxylin (or Gill 3) 10 dips
Scott’s water 10 dips
Eosin y alcoholic solution 10 dips
Absolute Ethanol 10 dips (for 3 times)
Clearing agent and mount
Results:
Nuclei………………………… purple
Cytoplasm…………………….Pink-red
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